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Addgene

pCMV6-LacZ-BbsI
(Plasmid #114671)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114671 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6
  • Backbone manufacturer
    Origene
  • Backbone size w/o insert (bp) 4508
  • Total vector size (bp) 4968
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Can likely be grown in other strains
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LacZ
  • Insert Size (bp)
    460
  • Promoter Lac promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BbsI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-LacZ-BbsI was a gift from Lynne Postovit (Addgene plasmid # 114671 ; http://n2t.net/addgene:114671 ; RRID:Addgene_114671)
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