pGL3-TK-5UTR-BsmBI-Luciferase
(Plasmid #114670)

• Purpose: For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter
• Depositing Lab: Lynne Postovit
• Publication: Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi: 10.1038/s41467-020-16352-z.

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4895
• Total vector size (bp): 5354
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Can likely grow in other strains as well
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LacZ
• Insert Size (bp): 459
• Promoter: lac promoter
• Tag / Fusion Protein:
  • for cloning 5' UTR-Luciferase fusions

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (not destroyed)
• 3′ cloning site: BsmBI (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry