pGEX-mE6-AP/2525
(Plasmid
#11467)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11467 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-KG
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Backbone manufacturersee PMID 1852137
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemE6-AP
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Alt nameUbe3a
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SpeciesM. musculus (mouse)
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Insert Size (bp)1500
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Mutationn/a
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GenBank IDU96636
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Entrez GeneUbe3a (a.k.a. 4732496B02, 5830462N02Rik, A130086L21Rik, Hpve6a)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hinc II/SmaI (destroyed during cloning)
- 3′ cloning site Sma i/Sma I (not destroyed)
- 5′ sequencing primer Amersham 5' pGEX
- 3′ sequencing primer Amersham 3' pGEX (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-mE6-AP/2525 was a gift from Allan Weissman (Addgene plasmid # 11467 ; http://n2t.net/addgene:11467 ; RRID:Addgene_11467) -
For your References section:
Subcellular localization and ubiquitin-conjugating enzyme (E2) interactions of mammalian HECT family ubiquitin protein ligases. Hatakeyama S, Jensen JP, Weissman AM. J Biol Chem. 1997 Jun 13. 272(24):15085-92. 10.1074/jbc.272.24.15085 PubMed 9182527