pcmvtag2Bmciap2
(Plasmid #11463)

• Depositing Lab: Jon Ashwell
• Publication: Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Backbone

• Vector backbone: pcmvtag2B
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mciap2
• Alt name: Birc3
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Entrez Gene: Birc3 (a.k.a. A, AW107670, Api1, Api2, Birc2, C-IAP2, HI, HIAP2, I, IAP1, IAP2, MI, MIAP1, MIAP2, MIH, MIHB, MIHC, RNF, RNF49, cI, cIA, cIAP-1, cIAP-2, cIAP1, cIAP2)
• Tag / Fusion Protein:
  • flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are several additional mutations in this construct - R398W, D419E, R445W and I591V. The depositing lab is unaware of any effects these mutations may have on plasmid function.