pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q
(Plasmid
#114617)
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PurposeBacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTWIN1
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Backbone manufacturerNEB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHuntingtin Exon1 fragment
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SpeciesH. sapiens (human)
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MutationA2C; 23 polyQ repeat
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Entrez GeneHTT (a.k.a. HD, IT15, LOMARS)
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Tag
/ Fusion Protein
- His6-Ssp DnaB intein (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-Term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q was a gift from Hilal Lashuel (Addgene plasmid # 114617 ; http://n2t.net/addgene:114617 ; RRID:Addgene_114617) -
For your References section:
Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths. Warner JB 4th, Ruff KM, Tan PS, Lemke EA, Pappu RV, Lashuel HA. J Am Chem Soc. 2017 Oct 18;139(41):14456-14469. doi: 10.1021/jacs.7b06659. Epub 2017 Oct 9. 10.1021/jacs.7b06659 PubMed 28937758
