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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C
(Plasmid #114610)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114610 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIN1
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Huntingtin Exon1 fragment
  • Species
    H. sapiens (human)
  • Mutation
    A2C, P90C; 15 polyQ repeat
  • Entrez Gene
    HTT (a.k.a. HD, IT15, LOMARS)
  • Tag / Fusion Protein
    • His6-Ssp DnaB intein (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C was a gift from Hilal Lashuel (Addgene plasmid # 114610 ; http://n2t.net/addgene:114610 ; RRID:Addgene_114610)

  • For your References section:

    Monomeric Huntingtin Exon 1 Has Similar Overall Structural Features for Wild-Type and Pathological Polyglutamine Lengths. Warner JB 4th, Ruff KM, Tan PS, Lemke EA, Pappu RV, Lashuel HA. J Am Chem Soc. 2017 Oct 18;139(41):14456-14469. doi: 10.1021/jacs.7b06659. Epub 2017 Oct 9. 10.1021/jacs.7b06659 PubMed 28937758
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