His6_BcLOV4_mCherry_BamUK
(Plasmid
#114596)
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PurposeEncodes mCherry-tagged BcLOV4 for expression in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneBamUK
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 7500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBcLOV4
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Alt nameBcRGS1
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SpeciesBotrytis cinerea
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Insert Size (bp)2500
- Promoter T7
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Tags
/ Fusion Proteins
- mCherry (C terminal on insert)
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7-forward
- 3′ sequencing primer T7-terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His6_BcLOV4_mCherry_BamUK was a gift from Brian Chow (Addgene plasmid # 114596 ; http://n2t.net/addgene:114596 ; RRID:Addgene_114596) -
For your References section:
Directly light-regulated binding of RGS-LOV photoreceptors to anionic membrane phospholipids. Glantz ST, Berlew EE, Jaber Z, Schuster BS, Gardner KH, Chow BY. Proc Natl Acad Sci U S A. 2018 Aug 14;115(33):E7720-E7727. doi: 10.1073/pnas.1802832115. Epub 2018 Jul 31. 10.1073/pnas.1802832115 PubMed 30065115
