BcLOV4-mammalian-recoded_mCherry_pcDNA3.1
(Plasmid #114595)

• Purpose: Encodes mCherry-tagged BcLOV4 for expression in mammalian cells
• Depositing Lab: Brian Chow
• Publication: Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;115(33):E7720-E7727. doi: 10.1073/pnas.1802832115. Epub 2018 Jul 31.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7900
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BcLOV4
• Alt name: BcRGS1
• Species: Botrytis cinerea
• Insert Size (bp): 2500
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry