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Addgene

pHMMa.PH1704
(Plasmid #11452)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11452 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHMMa
  • Backbone size w/o insert (bp) 3188
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    intracellular protease
  • Species
    P. horikoshii
  • Insert Size (bp)
    498
  • GenBank ID
    BAA30818 PH1704 NP_143548

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the plasmid pHMMa.PH1704 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHMMa.PH1704 was a gift from Sung-Hou Kim (Addgene plasmid # 11452 ; http://n2t.net/addgene:11452 ; RRID:Addgene_11452)

  • For your References section:

    Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution. Du X, Choi IG, Kim R, Wang W, Jancarik J, Yokota H, Kim SH. Proc Natl Acad Sci U S A. 2000 Dec 19. 97(26):14079-84. 10.1073/pnas.260503597 PubMed 11114201
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