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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11448 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBABE bleo
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Backbone manufacturerpBABE-bleo/zeo is available at Addgene
- Backbone size w/o insert (bp) 4888
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Vector typeMammalian Expression, Retroviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRS-1
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Alt nameInsulin Receptor Substrate 1
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Alt nameIRS1
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SpeciesH. sapiens (human)
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Insert Size (bp)3750
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GenBank IDNM_005544
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Entrez GeneIRS1 (a.k.a. HIRS-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBABE 5 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE bleo human IRS-1 was a gift from Ronald Kahn (Addgene plasmid # 11448 ; http://n2t.net/addgene:11448 ; RRID:Addgene_11448) -
For your References section:
Differential signaling by insulin receptor substrate 1 (IRS-1) and IRS-2 in IRS-1-deficient cells. Bruning JC, Winnay J, Cheatham B, Kahn CR. Mol Cell Biol. 1997 Mar . 17(3):1513-21. PubMed 9032279