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Addgene

pTriEX Tom20 mVenus BoLD
(Plasmid #114468)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEX
  • Backbone size w/o insert (bp) 5886
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BoLD
  • Species
    Synthetic
  • Insert Size (bp)
    168
  • Promoter CMV
  • Tags / Fusion Proteins
    • TOM20 and mVenus (N terminal on insert)
    • HIS, TEV and FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTriEX Tom20 mVenus BoLD was a gift from Andrew Woolley (Addgene plasmid # 114468 ; http://n2t.net/addgene:114468 ; RRID:Addgene_114468)

  • For your References section:

    Discovering Selective Binders for Photoswitchable Proteins Using Phage Display. Reis JM, Xu X, McDonald S, Woloschuk RM, Jaikaran ASI, Vizeacoumar FS, Woolley GA, Uppalapati M. ACS Synth Biol. 2018 Oct 19;7(10):2355-2364. doi: 10.1021/acssynbio.8b00123. Epub 2018 Sep 27. 10.1021/acssynbio.8b00123 PubMed 30203962
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