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Addgene

PLL7.0 tgRFP LOV
(Plasmid #114463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PLL 7.0
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LOV2
  • Insert Size (bp)
    480
  • Mutation
    G401A(G528A), N411E(N538E) and V289I (V416I)
  • Promoter CMV
  • Tag / Fusion Protein
    • tgRFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid lacks features necessary for lentiviral production, but should be unaffected for mammalian expression of tgRFP LOV.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLL7.0 tgRFP LOV was a gift from Andrew Woolley (Addgene plasmid # 114463 ; http://n2t.net/addgene:114463 ; RRID:Addgene_114463)

  • For your References section:

    Discovering Selective Binders for Photoswitchable Proteins Using Phage Display. Reis JM, Xu X, McDonald S, Woloschuk RM, Jaikaran ASI, Vizeacoumar FS, Woolley GA, Uppalapati M. ACS Synth Biol. 2018 Oct 19;7(10):2355-2364. doi: 10.1021/acssynbio.8b00123. Epub 2018 Sep 27. 10.1021/acssynbio.8b00123 PubMed 30203962
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