pMRXIP-GFP-hATG2A
(Plasmid #114462)

• Purpose: Expresses human ATG2A with GFP in mammalian cells
• Depositing Lab: Noboru Mizushima
• Publication: Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. doi: 10.1002/1873-3468.12901. Epub 2017 Nov 20.

Backbone

• Vector backbone: pMRXIP GFP-Ci2
• Backbone manufacturer: Dr.Shoji Yamaoka of Tokyo Medical and Dental University
• Backbone size w/o insert (bp): 6882
• Total vector size (bp): 12699
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human ATG2A
• Alt name: ATG2A
• Species: H. sapiens (human)
• Insert Size (bp): 5817
• Mutation: P656R (please see depositors comment below)
• GenBank ID: NM_015104
• Entrez Gene: ATG2A (a.k.a. BLTP4A)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TCTATCGATGAATTGGAATTCATGTCACGATGGCTG
• 3′ sequencing primer: TCGCGAACGCGTGAATTCTCAGTCTTGGGCACT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: It has the pMX backbone
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation P656R in hATG2A was found during Addgene's quality control process. The depositor has noted that this mutation is a natural variant, and does not affect plasmid function.