pIAGO-pegA1
(Plasmid
#114455)
-
PurposeExpression of PegA glycosyltansferase in streptomycetes
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 114455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepIAGO
-
Vector typeBacterial Expression
-
Selectable markersThiostrepton for streptomycete hosts
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepegA
- Promoter ermE
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl II (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer 5_ ATGCGAGTGTCCGTTCGAGTG 3_
- 3′ sequencing primer 5_ GTTAGCTCACTCATTAGGCAC 3_ (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pIAGO-pegA1 was a gift from Patrick Caffrey (Addgene plasmid # 114455 ; http://n2t.net/addgene:114455 ; RRID:Addgene_114455) -
For your References section:
Versatility of enzymes catalyzing late steps in polyene 67-121C biosynthesis. Stephens N, Rawlings B, Caffrey P. Biosci Biotechnol Biochem. 2013;77(4):880-3. doi: 10.1271/bbb.120961. Epub 2013 Apr 7. 10.1271/bbb.120961 PubMed 23563553