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Purpose(Empty Backbone) Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozyme
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114453 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS416
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Vector typeYeast Expression, CRISPR ; CEN/ARS
- Promoter ScGAL7p
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZS160 CRISPEY HDV-HDV NotI was a gift from Hunter Fraser (Addgene plasmid # 114453 ; http://n2t.net/addgene:114453 ; RRID:Addgene_114453) -
For your References section:
Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing. Sharon E, Chen SA, Khosla NM, Smith JD, Pritchard JK, Fraser HB. Cell. 2018 Sep 18. pii: S0092-8674(18)31118-8. doi: 10.1016/j.cell.2018.08.057. 10.1016/j.cell.2018.08.057 PubMed 30245013