pVL-FLAG/His LCOR
(Plasmid
#114450)
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PurposeInsect baculovirus expression vector containing N terminal FLAG/His tagged human LCOR coding sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114450 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepVL FLAG/His
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLCOR
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SpeciesH. sapiens (human)
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Insert Size (bp)1302
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Entrez GeneLCOR (a.k.a. C10orf12, MLR2)
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Tag
/ Fusion Protein
- FLAG-His (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVL-FLAG/His LCOR was a gift from Adrian Bracken (Addgene plasmid # 114450 ; http://n2t.net/addgene:114450 ; RRID:Addgene_114450) -
For your References section:
A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities. Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, Margueron R, Koseki H, Bracken AP. Mol Cell. 2018 May 3;70(3):408-421.e8. doi: 10.1016/j.molcel.2018.03.005. Epub 2018 Apr 5. 10.1016/j.molcel.2018.03.005 PubMed 29628311