pBS mouse IRS4 promoter
(Plasmid
#11445)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11445 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIRS-4 promoter
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Alt nameInsulin Receptor Substrate 4 promoter
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Alt nameIrs4 promoter
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Alt nameIrs4
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SpeciesM. musculus (mouse)
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Insert Size (bp)3000
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Entrez GeneIrs4 (a.k.a. IRS-4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS mouse IRS4 promoter was a gift from Ronald Kahn (Addgene plasmid # 11445 ; http://n2t.net/addgene:11445 ; RRID:Addgene_11445) -
For your References section:
Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling. Tsuruzoe K, Emkey R, Kriauciunas KM, Ueki K, Kahn CR. Mol Cell Biol. 2001 Jan . 21(1):26-38. 10.1128/MCB.21.1.26-38.2001 PubMed 11113178
Map uploaded by the depositor.
