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pLENTI EF1alpha FLAG/HA-LCOR
(Plasmid #114444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114444 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLENTI EF1_ FLAG/HA
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin ; Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LCOR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1302
  • Entrez Gene
    LCOR (a.k.a. C10orf12, MLR2)
  • Tag / Fusion Protein
    • FLAG-HA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLENTI EF1alpha FLAG/HA-LCOR was a gift from Adrian Bracken (Addgene plasmid # 114444 ; http://n2t.net/addgene:114444 ; RRID:Addgene_114444)

  • For your References section:

    A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities. Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, Margueron R, Koseki H, Bracken AP. Mol Cell. 2018 May 3;70(3):408-421.e8. doi: 10.1016/j.molcel.2018.03.005. Epub 2018 Apr 5. 10.1016/j.molcel.2018.03.005 PubMed 29628311
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