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PurposeEntry vector for gateway cloning containing human JARID2 coding sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114443 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR8/GW/TOPO
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Backbone manufacturerInvitrogen
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Vector typeGateway Entry Cloning
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJARID2
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SpeciesH. sapiens (human)
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Insert Size (bp)3741
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Entrez GeneJARID2 (a.k.a. DIDDF, JMJ)
- Promoter None
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR8 JARID2 was a gift from Adrian Bracken (Addgene plasmid # 114443 ; http://n2t.net/addgene:114443 ; RRID:Addgene_114443) -
For your References section:
A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities. Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, Margueron R, Koseki H, Bracken AP. Mol Cell. 2018 May 3;70(3):408-421.e8. doi: 10.1016/j.molcel.2018.03.005. Epub 2018 Apr 5. 10.1016/j.molcel.2018.03.005 PubMed 29628311