pBS human Frataxin (myc tag)
(Plasmid #11443)

• Depositing Lab: Ronald Kahn
• Publication: Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 97(22):12239-43.

Backbone

• Vector backbone: pBluescript II KS
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Frataxin
• Alt name: FRDA
• Species: H. sapiens (human)
• Insert Size (bp): 660
• Entrez Gene: FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
• Tag / Fusion Protein:
  • myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

(kahn lab plasmid name = FM Blue)

This plasmid was cloned before there was any consensus sequence for frataxin. There are 2 mutations with the currently available frataxin sequence - L140P R165C. The effect of these mutations is unknown.