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Addgene

2xsspB-mSc-sspB-Tiam1
(Plasmid #114424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114424 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pClontech-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tiam1
  • Species
    Synthetic
  • Mutation
    truncated Tiam1 (64-437)
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2xsspB-mSc-sspB-Tiam1 was a gift from Dorus Gadella (Addgene plasmid # 114424 ; http://n2t.net/addgene:114424 ; RRID:Addgene_114424)

  • For your References section:

    Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Van Geel O, Hartsuiker R, Gadella TWJ. Small GTPases. 2018 Sep 5:1-10. doi: 10.1080/21541248.2018.1507411. 10.1080/21541248.2018.1507411 PubMed 30182785
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