pAB(EXPR-HDAC5-trun1)
(Plasmid
#114400)
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PurposeFor PYL-HDAC5 aa 1-275 expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-CMV-GFP
- Backbone size w/o insert (bp) 4977
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePYL-HDAC5 aa 1-275
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SpeciesH. sapiens (human)
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Insert Size (bp)1511
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Entrez GeneHDAC5 (a.k.a. HD5, NY-CO-9)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFlag-NLS (internal)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAB(EXPR-HDAC5-trun1) was a gift from Vincent Dion (Addgene plasmid # 114400 ; http://n2t.net/addgene:114400 ; RRID:Addgene_114400) -
For your References section:
Expanded CAG/CTG repeats resist gene silencing mediated by targeted Epigenome editing. Yang B, Borgeaud AC, Buricova M, Aeschbach L, Rodriguez-Lima O, Ruiz Buendia GA, Cinesi C, Taylor AS, Baubec T, Dion V. Hum Mol Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255. 10.1093/hmg/ddab255 PubMed 34494094