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pAB(EXPR-DS-HDAC5_HDAC3)
(Plasmid #114399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CMV-GFP
  • Backbone size w/o insert (bp) 4974
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PYL-HDAC5_HDAC3 catalytic domain swap
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3962
  • Entrez Gene
    HDAC3 (a.k.a. HD3, KDAC3, RPD3, RPD3-2)
  • Entrez Gene
    HDAC5 (a.k.a. HD5, NY-CO-9)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag-NLS (internal)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAB(EXPR-DS-HDAC5_HDAC3) was a gift from Vincent Dion (Addgene plasmid # 114399 ; http://n2t.net/addgene:114399 ; RRID:Addgene_114399)

  • For your References section:

    Expanded CAG/CTG repeats resist gene silencing mediated by targeted Epigenome editing. Yang B, Borgeaud AC, Buricova M, Aeschbach L, Rodriguez-Lima O, Ruiz Buendia GA, Cinesi C, Taylor AS, Baubec T, Dion V. Hum Mol Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255. 10.1093/hmg/ddab255 PubMed 34494094
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