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Addgene

pAB-NEO-PYL
(Plasmid #114393)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114393 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CMV-GFP
  • Backbone size w/o insert (bp) 5241
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PYL
  • Species
    Synthetic
  • Insert Size (bp)
    1259
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG-SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAB-NEO-PYL was a gift from Vincent Dion (Addgene plasmid # 114393 ; http://n2t.net/addgene:114393 ; RRID:Addgene_114393)

  • For your References section:

    Expanded CAG/CTG repeats resist gene silencing mediated by targeted Epigenome editing. Yang B, Borgeaud AC, Buricova M, Aeschbach L, Rodriguez-Lima O, Ruiz Buendia GA, Cinesi C, Taylor AS, Baubec T, Dion V. Hum Mol Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255. 10.1093/hmg/ddab255 PubMed 34494094
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