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Addgene

pBabe bleo human PPAR gamma 2
(Plasmid #11439)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-zeo (aka: pBabe-bleo)
  • Backbone manufacturer
    pBabe-zeo is available at Addgene
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAR gamma 2
  • Alt name
    PPARg
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1525
  • Entrez Gene
    PPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5
  • 3′ sequencing primer pBABE-3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human PPARg2 (-10 to 1515 TAG) - insert was cut with XhoI and cloned into vector cut with SalI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe bleo human PPAR gamma 2 was a gift from Ronald Kahn (Addgene plasmid # 11439 ; http://n2t.net/addgene:11439 ; RRID:Addgene_11439)
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