pDONR-SUMO1
(Plasmid
#114388)
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PurposepDONR vector with SUMO1 gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114388 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneGateway Donor vector
- Backbone size w/o insert (bp) 2850
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSUMO1
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SpeciesH. sapiens (human)
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Insert Size (bp)306
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MutationQ29H compared with NCBI reference NP_003343.1
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Entrez GeneSUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13
- 3′ sequencing primer M13r (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR-SUMO1 was a gift from Vincent Dion (Addgene plasmid # 114388 ; http://n2t.net/addgene:114388 ; RRID:Addgene_114388) -
For your References section:
Minimizing carry-over PCR contamination in expanded CAG/CTG repeat instability applications. Aeschbach L, Dion V. Sci Rep. 2017 Dec 21;7(1):18026. doi: 10.1038/s41598-017-18168-2. 10.1038/s41598-017-18168-2 PubMed 29269788