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Addgene

pPN10-gDM1d
(Plasmid #114387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPN10
  • Backbone size w/o insert (bp) 5257
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gDM1d
  • gRNA/shRNA sequence
    TGCGAACCAACGATAGGTG
  • Insert Size (bp)
    5316

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPN10-gDM1d was a gift from Vincent Dion (Addgene plasmid # 114387 ; http://n2t.net/addgene:114387 ; RRID:Addgene_114387)

  • For your References section:

    Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase. Cinesi C, Aeschbach L, Yang B, Dion V. Nat Commun. 2016 Nov 9;7:13272. doi: 10.1038/ncomms13272. 10.1038/ncomms13272 PubMed 27827362
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