pPN10
(Plasmid
#114386)
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Purpose(Empty Backbone) Empty gRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 114386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPN10
- Backbone size (bp) 5257
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPN10 was a gift from Vincent Dion (Addgene plasmid # 114386 ; http://n2t.net/addgene:114386 ; RRID:Addgene_114386) -
For your References section:
Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase. Cinesi C, Aeschbach L, Yang B, Dion V. Nat Commun. 2016 Nov 9;7:13272. doi: 10.1038/ncomms13272. 10.1038/ncomms13272 PubMed 27827362