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pET-21a_2xNLS_LbCpf1_protein_expression
(Plasmid #114366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21 a
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 9705
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2xNLS_LbCpf1
  • Species
    Synthetic; Lachnospiraceae
  • Insert Size (bp)
    3778
  • Promoter T7
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-21a_2xNLS_LbCpf1_protein_expression was a gift from Scot Wolfe (Addgene plasmid # 114366 ; http://n2t.net/addgene:114366 ; RRID:Addgene_114366)

  • For your References section:

    Editing aberrant splice sites efficiently restores beta-globin expression in beta-thalassemia. Xu S, Luk K, Yao Q, Shen AH, Zeng J, Wu Y, Luo HY, Brendel C, Pinello L, Chui DHK, Wolfe SA, Bauer DE. Blood. 2019 Jan 31. pii: blood-2019-01-895094. doi: 10.1182/blood-2019-01-895094. 10.1182/blood-2019-01-895094 PubMed 30704988
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