pGEX-KG-Itch/2793
(Plasmid #11434)

• Depositing Lab: Allan Weissman
• Publication: Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98(25):14422-7.

Backbone

• Vector backbone: pGEX-KG
• Backbone manufacturer: see PMID 1852137
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Itch
• Alt name: Itchy
• Species: M. musculus (mouse)
• Insert Size (bp): 2500
• Mutation: n/a
• Entrez Gene: Itch (a.k.a. 6720481N21Rik, 8030492O04Rik, A130065M08, AIP4, C230047C07Rik)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ? (unknown if destroyed)
• 3′ cloning site: ? (unknown if destroyed)
• 5′ sequencing primer: Amersham 5' pGEX
• 3′ sequencing primer: Amersham 3' pGEX

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Neal Copeland/ NCI

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry