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pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry
(Plasmid #114322)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114322 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCLX
  • Modifications to backbone
    This plasmid allows for 2-plasmid Gateway cloning of a gene of interest in pENTRL1L2. It is a all-in-one auto-inducible lentivector using Mifepristone as drug inducer and mCherry as live tracker of transduced cells independently of drug induction.
  • Vector type
    Lentiviral
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry was a gift from Patrick Salmon (Addgene plasmid # 114322 ; http://n2t.net/addgene:114322 ; RRID:Addgene_114322)

  • For your References section:

    Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior. Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ. Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w. 10.1038/s41467-017-01046-w PubMed 29079819
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