pCWX-R4DESTR2-PGK-Tk:Sh
(Plasmid
#114321)
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Purpose(Empty Backbone) Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Zeocin/Bleomycin resistance and Acyclovir conditional ablation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114321 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCWX
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Vector typeLentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid allows for 3-plasmid Gateway cloning of a gene of interest with a promoter of choice. GOI is in pENTRL1L2 and promoter is in pENTRL4L1R
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCWX-R4DESTR2-PGK-Tk:Sh was a gift from Patrick Salmon (Addgene plasmid # 114321 ; http://n2t.net/addgene:114321 ; RRID:Addgene_114321)