pCLXEBR-pTF-kRasV12
(Plasmid #114318)

• Purpose: TET-inducible expression of the V12-mutant form of the ki-Ras protein
• Depositing Lab: Patrick Salmon
• Publication: Salmon Lab plasmids (unpublished)

Backbone

• Vector backbone: pCLXEBR65
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ras
• Species: H. sapiens (human)
• Mutation: G12V oncogenic mutation. Constitutively active K-ras protein.
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions. Subcloned from Addgene Plasmid #31200. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.