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pCWX-UBI-hTert-PGK-BSD
(Plasmid #114316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114316 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCWX-PGK-BSD
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hTERT
  • Species
    H. sapiens (human)
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCWX-UBI-hTert-PGK-BSD was a gift from Patrick Salmon (Addgene plasmid # 114316 ; http://n2t.net/addgene:114316 ; RRID:Addgene_114316)

  • For your References section:

    Generation of human cell lines using lentiviral-mediated genetic engineering. Salmon P. Methods Mol Biol. 2013;945:417-48. doi: 10.1007/978-1-62703-125-7_25. 10.1007/978-1-62703-125-7_25 PubMed 23097121
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