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Addgene

pCLXEBR-pTF-Ngn3
(Plasmid #114307)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114307 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCLXEBR65
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ngn3
  • Species
    H. sapiens (human)
  • Entrez Gene
    NEUROG3 (a.k.a. Atoh5, Math4B, NGN-3, bHLHa7, ngn3)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLXEBR-pTF-Ngn3 was a gift from Patrick Salmon (Addgene plasmid # 114307 ; http://n2t.net/addgene:114307 ; RRID:Addgene_114307)
  • For your References section:

    Polyswitch lentivectors: "all-in-one" lentiviral vectors for drug-inducible gene expression, live selection, and recombination cloning. Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P. Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27. 10.1089/hum.2010.179 PubMed 21761975