pCLXEBR-pTF-Ngn3
(Plasmid
#114307)
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PurposeTET-inducible expression of the Neugenin 3 transcription factor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114307 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCLXEBR65
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNgn3
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SpeciesH. sapiens (human)
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Entrez GeneNEUROG3 (a.k.a. Atoh5, Math4B, NGN-3, bHLHa7, ngn3)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCLXEBR-pTF-Ngn3 was a gift from Patrick Salmon (Addgene plasmid # 114307 ; http://n2t.net/addgene:114307 ; RRID:Addgene_114307) -
For your References section:
Polyswitch lentivectors: "all-in-one" lentiviral vectors for drug-inducible gene expression, live selection, and recombination cloning. Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P. Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27. 10.1089/hum.2010.179 PubMed 21761975