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Addgene

pCWXPG-UBI-Ngn3PDX1MafA
(Plasmid #114303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCWXPG
  • Vector type
    Lentiviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ngn3
  • Species
    H. sapiens (human)
  • Mutation
    Ngn3, PDX1 and MafA proteins are expressed from a precursor with self-cleavable 2A peptides
  • Entrez Gene
    NEUROG3 (a.k.a. Atoh5, Math4B, NGN-3, bHLHa7, ngn3)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCWXPG-UBI-Ngn3PDX1MafA was a gift from Patrick Salmon (Addgene plasmid # 114303 ; http://n2t.net/addgene:114303 ; RRID:Addgene_114303)

  • For your References section:

    Polyswitch lentivectors: "all-in-one" lentiviral vectors for drug-inducible gene expression, live selection, and recombination cloning. Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P. Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27. 10.1089/hum.2010.179 PubMed 21761975
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