pCLX-M38s/TOP-dGFP
(Plasmid
#114295)
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PurposeExpression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCLX
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedGFP
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Mutationdestabilized GFP
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Subcloned from Addgene Plasmid #17114
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCLX-M38s/TOP-dGFP was a gift from Patrick Salmon (Addgene plasmid # 114295 ; http://n2t.net/addgene:114295 ; RRID:Addgene_114295) -
For your References section:
Wnt signaling regulates multipolar-to-bipolar transition of migrating neurons in the cerebral cortex. Boitard M, Bocchi R, Egervari K, Petrenko V, Viale B, Gremaud S, Zgraggen E, Salmon P, Kiss JZ. Cell Rep. 2015 Mar 3;10(8):1349-61. doi: 10.1016/j.celrep.2015.01.061. Epub 2015 Feb 26. 10.1016/j.celrep.2015.01.061 PubMed 25732825