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Addgene

pCWXPGR-pTF-ERK:MEK
(Plasmid #114291)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114291 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCWXPGR65
  • Vector type
    Lentiviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rERK2 and hMEK1 proteins
  • Species
    H. sapiens (human), R. norvegicus (rat)
  • Mutation
    rat ERK2 fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES
  • Entrez Gene
    Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

rat ERK2 fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES. Subcloned from Addgene plasmid #39197. pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCWXPGR-pTF-ERK:MEK was a gift from Patrick Salmon (Addgene plasmid # 114291 ; http://n2t.net/addgene:114291 ; RRID:Addgene_114291)
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