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pGEX-gp78c/2767
(Plasmid #11429)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11429 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T2
  • Backbone manufacturer
    Amersham/GE
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gp78c
  • Alt name
    AMFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    n/a
  • GenBank ID
    AF124145
  • Entrez Gene
    AMFR (a.k.a. GP78, RNF45, SPG89)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer Amersham 5' pGEX
  • 3′ sequencing primer Amersham 3' pGEX
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A Raz, PMID 96466414

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-gp78c/2767 was a gift from Allan Weissman (Addgene plasmid # 11429 ; http://n2t.net/addgene:11429 ; RRID:Addgene_11429)

  • For your References section:

    The tumor autocrine motility factor receptor, gp78, is a ubiquitin protein ligase implicated in degradation from the endoplasmic reticulum. Fang S, Ferrone M, Yang C, Jensen JP, Tiwari S, Weissman AM. Proc Natl Acad Sci U S A. 2001 Dec 4. 98(25):14422-7. 10.1073/pnas.251401598 PubMed 11724934
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