-
PurposeTET-inducible expression of a constitutively active form of the BetaCatenin protein
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 114281 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCWXPGR65
-
Vector typeLentiviral
-
Selectable markersGFP
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCatenin beta 1
-
SpeciesH. sapiens (human)
-
Mutationdeletion of AA 1 to 45
-
Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
-
Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCWXPGR-pTF-betaCatenin was a gift from Patrick Salmon (Addgene plasmid # 114281 ; http://n2t.net/addgene:114281 ; RRID:Addgene_114281) -
For your References section:
Wnt signaling regulates multipolar-to-bipolar transition of migrating neurons in the cerebral cortex. Boitard M, Bocchi R, Egervari K, Petrenko V, Viale B, Gremaud S, Zgraggen E, Salmon P, Kiss JZ. Cell Rep. 2015 Mar 3;10(8):1349-61. doi: 10.1016/j.celrep.2015.01.061. Epub 2015 Feb 26. 10.1016/j.celrep.2015.01.061 PubMed 25732825