pCINeo-myc-Itch (C822S)/2860
(Plasmid #11428)

• Depositing Lab: Allan Weissman
• Publication: Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-77.

Backbone

• Vector backbone: pCI-Neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Itch
• Alt name: Itchy
• Species: M. musculus (mouse)
• Insert Size (bp): 2500
• Mutation: cysteine at 822 mutated to serine
• GenBank ID: AF037454
• Entrez Gene: Itch (a.k.a. 6720481N21Rik, 8030492O04Rik, A130065M08, AIP4, C230047C07Rik)
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe I (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: T7 (modified) 5'-TAATACGACTCACTATAGG-3'
• 3′ sequencing primer: T3 (common)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Neal Copeland/NCI
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry