pCWXPGR-pTF-dnTCF4
(Plasmid
#114277)
-
PurposeTET-inducible expression of a dominant negative form of the TCF4 transcription factor
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 114277 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCWXPGR65
-
Vector typeLentiviral
-
Selectable markersGFP
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTCF4
-
SpeciesH. sapiens (human)
-
Mutationdeletion of AA 1 to 31
-
Entrez GeneTCF4 (a.k.a. CDG2T, E2-2, FCD2, FECD3, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, SEF2-1D, TCF-4, bHLHb19)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCWXPGR-pTF-dnTCF4 was a gift from Patrick Salmon (Addgene plasmid # 114277 ; http://n2t.net/addgene:114277 ; RRID:Addgene_114277) -
For your References section:
Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior. Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ. Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w. 10.1038/s41467-017-01046-w PubMed 29079819