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Addgene

pCWXPGR-pTF-DVL2deltaDIX
(Plasmid #114276)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114276 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCWXPGR65
  • Vector type
    Lentiviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DVL2
  • Species
    M. musculus (mouse)
  • Mutation
    deletion of DIX domain
  • Entrez Gene
    Dvl2
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCWXPGR-pTF-DVL2deltaDIX was a gift from Patrick Salmon (Addgene plasmid # 114276 ; http://n2t.net/addgene:114276 ; RRID:Addgene_114276)
  • For your References section:

    Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior. Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ. Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w. 10.1038/s41467-017-01046-w PubMed 29079819