Col2.3deltaTK
(Plasmid #114273)

• Purpose: Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.
• Depositing Lab: David Rowe
• Publication: Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-31. doi: 10.1359/jbmr.2001.16.12.2222.

Backbone

• Vector backbone: pBR327
• Backbone size w/o insert (bp): 1850
• Total vector size (bp): 5700
• Modifications to backbone: Tet resistance gene deleted.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Col2.3 promoter + deltaTK + BGH polyA
• Species: R. norvegicus (rat), B. taurus (bovine); Herpes simplex virus
• Insert Size (bp): 3775
• Promoter: 2.3 kb rat type 1 collagen

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: rColprom-121F
• 3′ sequencing primer: bGHpA-95R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Delta TK gene was a gift from Dr. David Klatzmann, Laboratoire de Biologie et The´rapeutique des Pathologies Immunitaires, Centre National de la Recherche Scientifique ERS 107, Hoˆpital de la Pitie´-Salpeˆtrie`re, 75651 Paris Cedex 13, France

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry