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Addgene

pMLS415
(Plasmid #114272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR-221
  • Backbone manufacturer
    Thermofisher
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 4800
  • Vector type
    Cre/Lox, CRISPR
  • Selectable markers
    Unc-119

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cbr-Unc-119
  • Species
    Caenorhabditis briggsae
  • Insert Size (bp)
    2274
  • Mutation
    *please see depositor comment below

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: the Cbr-unc-119 rescue cassette contains 3 point mutations that do not affect unc-119 rescue, either when present as an array or as a single copy insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMLS415 was a gift from Erik Jorgensen (Addgene plasmid # 114272 ; http://n2t.net/addgene:114272 ; RRID:Addgene_114272)

  • For your References section:

    SapTrap vectors for introducing point mutations with unc-119+ selection. Schwartz M, Jorgensen E. MicroPubl Biol. 2018 Jul 29;2018:10.17912/DDVH-BG64. doi: 10.17912/DDVH-BG64. 10.17912/DDVH-BG64 PubMed 32550388
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