pMLS253
(Plasmid
#114271)
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PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR-221
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 4800
- Total vector size (bp) 4800
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Vector typeCre/Lox, CRISPR
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Selectable markersUnc-119
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCbr-Unc-119
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SpeciesCaenorhabditis briggsae
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Insert Size (bp)2279
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Mutation*please see depositor comment below
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: the Cbr-unc-119 rescue cassette contains 3 point mutations that do not affect unc-119 rescue, either when present as an array or as a single copy insert.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMLS253 was a gift from Erik Jorgensen (Addgene plasmid # 114271 ; http://n2t.net/addgene:114271 ; RRID:Addgene_114271) -
For your References section:
SapTrap vectors for introducing point mutations with unc-119+ selection. Schwartz M, Jorgensen E. MicroPubl Biol. 2018 Jul 29;2018:10.17912/DDVH-BG64. doi: 10.17912/DDVH-BG64. 10.17912/DDVH-BG64 PubMed 32550388
Map uploaded by the depositor.
