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pCINeo-myc-Itch/2859
(Plasmid #11427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Itch
  • Alt name
    Itchy
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • Mutation
    n/a
  • GenBank ID
    AF037454
  • Entrez Gene
    Itch (a.k.a. 6720481N21Rik, 8030492O04Rik, A130065M08, AIP4, C230047C07Rik)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Neal Copeland/NCI
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains ~1kb of 3' UTR of the mouse Itch gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCINeo-myc-Itch/2859 was a gift from Allan Weissman (Addgene plasmid # 11427 ; http://n2t.net/addgene:11427 ; RRID:Addgene_11427)

  • For your References section:

    WW domain HECT E3s target Cbl RING finger E3s for proteasomal degradation. Magnifico A, Ettenberg S, Yang C, Mariano J, Tiwari S, Fang S, Lipkowitz S, Weissman AM. J Biol Chem. 2003 Oct 31. 278(44):43169-77. 10.1074/jbc.M308009200 PubMed 12907674
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