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Addgene

pBlueBac human APC2
(Plasmid #11425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueBac His2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4900
  • Vector type
    baculoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2600
  • GenBank ID
    BC032503
  • Entrez Gene
    ANAPC2 (a.k.a. APC2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SphI (unknown if destroyed)
  • 5′ sequencing primer Polyhedrin forward (AAATGATAACCATCTCGC)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

full length, wild-type baculoviral expression of hAPC2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBlueBac human APC2 was a gift from Marc Kirschner (Addgene plasmid # 11425 ; http://n2t.net/addgene:11425 ; RRID:Addgene_11425)

  • For your References section:

    Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Yu H, Peters JM, King RW, Page AM, Hieter P, Kirschner MW. Science. 1998 Feb 20. 279(5354):1219-22. 10.1126/science.279.5354.1219 PubMed 9469815
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