pBS aP2 promoter (5.4kb) polyA
(Plasmid #11424)

• Depositing Lab: Ronald Kahn
• Publication: aP2 promoter (unpublished)

Backbone

• Vector backbone: pBluescript SK II +
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: aP2 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 5400
• GenBank ID: M84651
• Entrez Gene: Fabp4 (a.k.a. 422/aP2, AFABP, ALBP, ALBP/Ap2, Ap2, Lbpl, P15)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Addgene Notes:
  • Compiled sequence of plasmid 11424
• A portion of this plasmid was derived from a plasmid made by: Dr. Bruce Spiegelman
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PolyA PCR'd from pSG5 and inserted into the NotI and SacII sites of the plasmid pBS aP2 promoter (5.4kb). The polyA sequence is approximately 250bp and encompasses an additional EcoRI site.

An EcoRI site is also present at -1.7 kb in the aP2 promoter.

Addgene has compiled full sequence for this plasmid. Please see Notes link.