AAV-hSyn-EGFP-8X2C (AAV GABA mAGNET)
(Plasmid
#114214)
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PurposeAAV vector that targets expression to GABAergic inhibitory neurons in rodent cortex with 98% selectivity
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
- Total vector size (bp) 5780
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Vector typeMammalian Expression, Mouse Targeting, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEnhanced Green Fluorescent Protein
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Alt nameEGFP
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SpeciesM. musculus (mouse)
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Insert Size (bp)717
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MutationNone
- Promoter human synapsin (hSyn)
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied sequence. The authors have noted that the discrepancies do NOT affect plasmid function or viral packaging.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-hSyn-EGFP-8X2C (AAV GABA mAGNET) was a gift from Xue Han (Addgene plasmid # 114214 ; http://n2t.net/addgene:114214 ; RRID:Addgene_114214) -
For your References section:
A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex. Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X. Cell Rep. 2018 Jul 10;24(2):294-303. doi: 10.1016/j.celrep.2018.06.049. 10.1016/j.celrep.2018.06.049 PubMed 29996091